The customised 110 Defender, which belongs to Chris Burns of Breezeleaf Spaniels, Powder Mills, Leigh contained three springer spaniels and two cocker spaniels.
The dogs are all micro-chipped and contained in a silver box divided into three kennels on the back of the vehicle.
The vehicle’s number plate is DOG 9K.
The theft took place between 4.35pm and 5pm on Friday.
Anyone with information is urged to contact police on 01732 379217 or Kent Crimestoppers on 0800 555111.